| 摘要 |
<p>PURPOSE:To judge the freeze resistance of yeast in an extremely short period securely, by cultivating a yeast to be tested, freezing the crude fermented substance of the yeast in a germination state, preserving it for a fixed period, thawing it, detecting the live ratio of the yeast to be tested. CONSTITUTION:A yeast to be tested is fermented in a medium containing a saccharide such as sucrose, glucose, etc., the crude fermented substance of the yeast wherein the yeast to be tested in a germination state is frozen at -35- -45 deg.C, preferably at -40 deg.C, preserved for about 1-150hr, preferably 1-4days, thawed, and the live ratio of the yeast to be tested is detected by using a methylene blue dyed solution.</p> |
