摘要 |
PURPOSE:A specific microorganism in Candida is made to act on renewable fat or oil under aerobic conditions to produce directly a long-chain dicarboxylic acid in high yield. CONSTITUTION:The micoorganism used is a yeast in Candida, e.g., Candida tropicalis 1098, MD-105 and BR-254, while the fat or oil may be either vegetable one or animal one. A strain is inoculated directly to a culture medium containing the fat or oil as a substrate or cell bodies that are obtained by previously culturing the strain in a medium containing a carbon source the strain can assimilate are made to act on the culture medium containing the fat or oil. The amount of the fat or oil to be added is 5-40% and the cultivation is conducted at 25-35 deg.C aerobically to give the objective long-chain dicarboxylic acid. |