METHOD FOR PRODUCTION OF ANTISERUM AGAINST GRANULA FRACTION FROM ENDOCRINOLOGICALLY ACTIVE CELLS

摘要

Method of manufacturing antiserum against a peptide or protein component from endocrinally active cells for the identification of such cells by applying the antiserum to microscopic sections. Tissue with said endocrinally active cells is disintegrated, and from the disintegrated product an insoluble granule fraction including said peptide or protein component, is separated in a manner known per se by centrifugation and filtration steps. The insoluble granule fraction is suspended in a water solution, and the antiserum is manufactured by using the water suspension. The invention relates particularly to antisera against a granule fraction from tumours, particularly substance P producing carcinoid.