CROSSBRED DNA AND LINKABLE COMPOSITION PREPARED THEREBY

摘要

Transformed expression vectors or plasmids carry a ds DNA sequence that codes for only a desired part of a polypeptide chain of a protein or enzyme and is equipped for initiation and termination codons at the 5 min - and 3 min -termini respectively of said DNA sequence; in particular they carry a ds DNA sequence that codes for a variable region of a light or heavy chain of an immunoglobulin specific for a predetermined ligand but lacks nucleotides coding for aminoacid residues superfluous to said variable region. Methods for their preparation and hosts transformed by them are also disclosed. Cultivation of such transformed hosts yields the desired polypeptides, which can be isolated. Preferred polypeptides are provided in the form of compositions containing the variable regions of the light and heavy chains of immunoglobulins, since these are useful especially for diagnosis and therapy.