POLYNUCLEOTIDE SYNTHESIS

摘要

A process for the preparation of polynucleotides, in which phosphorus-oxygen bonds are formed at elevated temperature. The invention also provides an automated synthesiser for performing the process which uses Peltier effect semiconductor devices to provide the requisite elevated temperature and to cool the reagents to provide optimum conditions for the deprotection steps of a polynucleotide synthesis. The proces preferably involves the use of a mixture of benzen sulphonic acid and dimethylformamide as a deprotecting agent, in the absence of solvent alcohol.