Messenger RNA, production and use thereof.

摘要

The present invention provides a novel human Interleukin 2-encoding messenger RNA, a method of producing the same from the cells which are capable of producing human Interleukin 2 and a method of producing human Interieukin 2 using the same in an in vitro protein synthesis system.

The invention also provides a method of producing an intron-less complementary DNA copy of human Interieukin 2- encoding messenger RNA for insertion into an appropriate expression plasmid and introduction into appropriate host bacterial cells. Cultivation of the host produces highly pure Interleukin-2 in large amounts.