| 摘要 |
PURPOSE:To measure human villous gonadotropin (hCG) accurately in high sensitivity without affected by the interferrence by another similar hormone (e.g. human luteinizing hormone), by using beta-D-galactosidase as a labeling agent and using combined material combinding said labeling agent and an antibody with N,N'-O-phenylene dimaleimide. CONSTITUTION:An object containing hCG to be measured is added to an antibody held on a carrier and antigen.antibody reaction is carried out. Hereafter, the material combined beta-D-galactosidase obtained by said reaction with anti-hCG antibody IgG or Fab' is added and reacted. Then, 4-methyl umbelliferyl-beta-D- galactoside, etc. is added to this reaction product as a substrate of beta-D-galactosidase and activity of enzyme of a reaction product is found by measuring isolated 4-methyl umbelliferone, etc. by a fluorophotometer. The content of the hCG in the object is measured by applying fluorescence intensity obtained by the object containing unknown quantity of the hCG to a standard curve. |
