| 摘要 |
<p>An improved specific binding assay, e.g., homogeneous or heterogeneous immunoassay, for determining an analyte employing a labeled conjugate of said analyte and a photogenic, e.g., fluorescent, label. Where the analyte in the analyte-photophor conjugate whose light emission is measured in the assay as a function of the analyte is capable of significantly reducing such light emission upon coming into quenching orientation with the photophor, the present invention employs a labeled conjugate in which the analyte is joined to the photogenic label by a linking group which substantially hinders such quenching orientation. In this manner, ten percent or more of the photogenicity of the unconjugated photophor can be preserved in the measured analyte-photophor conjugate. The use of conventional flexible linking groups in the labeled conjugate allows quenching to occur with attendant large losses in assay sensitivity, whereas sensitivity is increased in the present invention by the use of relatively rigid linking groups. The invention is applicable to a wide variety of photogenic, e.g., fluorescent and chemiluminescent, binding assay techniques.</p> |
