| 摘要 |
PURPOSE:To improve the antibody activity and light emission intensity of marked antibodies by binding many luminescents with a polymer compd. having high bonding property with chemical luminescents and having high solubility in water, and binding many antibody fragments therewith. CONSTITUTION:Many compds. which prduce chemical luminescents such as luminol, lucigenin, lophin and the like are known, and explanation is given by taking an example of luminol. Luminol can be diazo-bound easily with antibodies by diazonizing the amino groups in 5 positions and can be bound with other proteins easily as well. Fab' phenylene dimaleimide bound bodies are mixed with the liquid fragments of the antihuman IgG obtd. by a known method, whereby the Fab'-albumin luminol bound bodies coupled with Fab' of >=2 molecules in each one molecule of alubmin are obtained. Such bound bodies have better antibody activity and quantity of light emission as compared to antibody-luminol bound bodies. |
