| 摘要 |
<p>The present invention discloses multi-functional recombinant DNA cloning vectors comprising
<UnorderedLists id="ula01" listStyle="none"><ListItem>a) two or more functionally different origins of replication that are indpendently selected from the group consisting of any origin of replication that is functional in Streptomyces, any origin of replication that is functional in Bacillus, and any origin of replication that is functional in E. coli, and</ListItem><ListItem>b) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a sensitive host cell in which an origin of replication comprising said vector is functional, said host cells being susceptible to transformation, cell division, and culture,</ListItem></UnorderedLists>
subject to the limitation that when. said vector is limited to two functionally different origins of replication, neither of the origins of replication is functional in E. coli.</p> |
