| 摘要 |
PURPOSE:To kill specific cells efficiently and to make the killing helpful for biochemical inspection by using the three; the biotinylated body having activity for inhibiting synthesis of specific non-cellular protein, the biotinylated body such as antibody that recognizes the specific cells and avidin. CONSTITUTION:The three; the biotinylated body of the antibody (anti Thy121gM which is the monoclonal antibody against the lymph cell antigen Thy12 of mouse) that recognizes specific cells (for example, lymphoma cells of mouse), the biotinylated body of protein (luffin) having activity for inhibiting synthesis of non-cellular protein extracted from the seeds of snake gourds and avidin (basic sugar protein existing in egg white and having high affinity to biotinyn) are added to cell groups (culturing media, etc.) contg. the above-described specific cells. Then in the case of the absence of complement fixability in antibodies in cell killing using antibodies and complements as in the prior art and without defects that the cell killing is disturbed by the components in the blood serum used as complement sources, the selective and efficient killing of only the specific cells is made possible. |
