ACID EXTRACTION OF BACTERIAL ENDOTOXIN FOR ASSAY BY LIMULUS TEST

摘要

A method for determining a bacterial endotoxin in an assay sample by an amoebocyte lysate component of horseshoe crab in the presence or absence of a substrate for endotoxin determination, characterized by using an assay sample in the liquid state obtained by treating said assay sample with an endotoxin-free acid having a pKa<25 DEG C> value of not more than 3 at a pH of not more than 3; and a kit therefore consisting essentially of a receptacle containing (a) an endotoxin-free acid having a pKa<25 DEG C> value of not more than 3 for treating the assay sample and a receptacle containing (b) an amoebocyte lysate component of horseshoe crab.