摘要 |
PURPOSE:Lymphocytes are sensitized with cancer cell-originating glucrose-relating antigens (GRA) to produce the titled lymphocytes, killer T-cells. CONSTITUTION:Constituents of cell membranes are separated from cancer cells having GRA such as human or animal's cultivated cancer cells, transplanted cancer cells, spontaneous cancer cells, a chemical substance or virus-generating cancer cells and treated with a lectin that specifically bonds with terminal galactoses such as peanut lectin or castor seed lectin to separate GRA. Lymphocytes are cultured in a medium containing the separated GRA at a pH near 7.2 and about 37 deg.C for 1-10 days to obtain killer T-cells. The resultant T-cells can be infinitely propagated, as its activity is being retained, in the above culture medium containing the T-cell propagating factors. The T-cells are preferably prepared into an injection solution together with a support and its concentration is 10<5>-10<12> cells/ml. The dose is preferably 10<5>-10<12> cells/kg. body weight/ day and it is given in portion, once to several times. |