| 摘要 |
PURPOSE:To measure enzyme immunity by adding respective solns. of ANS and oxalate to a soln. contg. hydrogen peroxide and measuring the quantity of the light from the mixed reacting solns. CONSTITUTION:A buffer of about 8-9.5 (for example, barbituric buffer) which is dissolved therein with a soln. contg. a luminous material 3-anilino-1-naphthalene sulfonic acid (ANS) and an org. solvent (for example, an ethyl acetate soln.) dissolved with oxalate (for example, bisoxalate) is added to the reacting liquid contg. hydrogen peroxide obtd. by a measuring system of an ordinary enzyme immunity method using various kinds of oxidases, for example, gluclose oxidase, galactose oxidase, colesterol oxidase as marking enzyme. According to this method, the ANS per se acts as a luminous material of a peroxyoxalate light emission method, whereby superior results are obtained. |
