| 摘要 |
A process for recovering L-proline from Chlorella sp. 580 algae without disrupting the L-proline synthesizing capability of the algae thereby permitting reuse of the proline depleted cells to produce additional amounts of L-proline. The process comprises cultivating Chlorella sp. 580 algae under high-intensity illumination in an aqueous growth medium containing a high concentration of sodium chloride (at least 1 M in the final stage of cultivation), providing an adequate supply of carbon, in a depth not exceeding approximately 20 cm of the aqueous medium, until algae of high L-proline content are obtained, harvesting the algae and thereafter diluting the harvested algae with water to a concentration below at least 0.3 M NaCl to effect the release of L-proline from the algae into the aqueous phase, removing the algae from the L-proline containing aqueous solution and reintroducing the algae into a high salt concentration containing medium of up to at least 1 M NaCl to restimulate L-proline production within the algae.
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