UN PROCEDIMIENTO PARA PREPARAR INSULINA HUMANA O DERIVADOS DE TREOMINA B30 DE INSULINA HUMANA.

摘要

A process for converting human des-B30-insulin, namely I into h-In-ThrB30 esters through amidation with an L-threonine ester in a mixture of water and a water miscible solvent in the presence of trypsin and optionally an acid. Yields in excess of at least 90% are obtained by limiting water content to 10%-30% v/v of the reaction mixture.