| 摘要 |
PURPOSE:To obtain a simple measuring method having high practical value without being interferred with temperature completely in normal operation temperature, by basing on a HbA1a, b, c batch elution using a micro column and passing a development liquid having lower salt concentration or pH than a buffer solution filled in the column preliminarily and then, forming a continuous concentration gradient in the column. CONSTITUTION:A column 1 packed with a weak acidic cation exchange resin 2 equillibrated by a phosphate buffer solution consisting of acidic phosphate and basic phosphate, is used. At first, caps 7 and 8 of the column 1 shown by figure (a) are taken off and the buffer solution in the column 1 is flowed out. Next, hemolytic blood 3 is dropped to the center of 50mul resin as shown by figure (b). After the blood 3 soaked into the resin, 750mul development solution 9 belonging to the same group as the buffer solution used for conditioning of the ion exchange resin and having lower hemoglobin elution than said buffer solution, is added to the blood 3 and the hemoglobin in the blood 3 is developed in the column 1 as shown by figure (c). HbA1a, b, c are eluted passing through 8ml eluting solution 10 having a higher hemoglobin elution effect than the solution 9 after throwing away the whole outflow from the lower end of the column 1. |
