DIACETYLDICHLOROFLUORESCIN WITH A SOURCE OF HYDROGEN PEROXIDE FOR ASSAY OF PEROXIDASE

摘要

An assay for substances demonstrating peroxidative activity is based on the peroxidase catalyzed oxidation of dichlorofluoresein to dichlorofluorescein by peroxide. The dichlorofluoresein is formed in situ from diacetyldichlorofluoresein either by the direct addition of peroxide or by the inclusion of a peroxide generating system in the reaction mixture.