| 摘要 |
<p>A method for the analysis of triglycerides in a biological fluid which comprises; (a) treating the biological fluid to remove endogenous glycerol and pyruvate by combining with the fluid, as a first component, adenosine triphosphate (ATP), enzyme glycerol kinase (GK) and a metal cation activator for the GK, as a second component, enzyme pyruvate kinase (PK) in the presence of substrate phosphoenol pyruvate (PEP) and an enzyme PK activator, and as a third component, glutamate and enzyme alanine aminotransferase (GPT); (b) enzymatically hydrolyzing the triglycerides in the resulting fluid free of glycerol and pyruvate by combining the fluid with a mixture comprised of a microbial lipase, a surfactant selected from polyoxyethylene (POE) alkylamides, POE esters of fatty acids, POE mercaptans, POE alkylamines, POE polyol esters, POE acetylenic glycols and POE phosphate esters, and an alkaline earth metal cation, in the form of a salt; and (c) determining the amount of glycerol produced from the enzymatic hydrolysis. The method disclosed obviates the need for parallel blank correction.</p> |
