| 摘要 |
PURPOSE:To quantitatively determine hemoglobin simply and quickly, by acting an oxidizing agent to the blood to produce Fe<+3> and denaturated protein, then reduced to Fe<+2> by adding a reducing agent and develops the color of Fe<+2> by a specific reagent, then dissolving denatured protein by adding a buffer solution, etc. and determine Fe<+2> by colorimetry. CONSTITUTION:A fixed quantity of 100 times diluted blood is sampled to a test tube and a NaClO solution, an aqueous solution of H2O2, and a mixed sulution of aqueous solution of Na2O2 etc., and hydrochloric acid, are added and is left for a prescribed time. Hereafter, an aqueous solution of a reducing agent such as ascorbic acid and thioglycolic acid, etc. to produce Fe<+2>. Next, after adding an aqueous solution of bathophenanthrone, 1M acetic acid buffer solution (6.5pH) or alkaline solution is added in order to remove turbidity of colored liquid and denatured protein is dissolved. Then, the colorimetric determination is carried out by 535nm wavelength. On one hand, blank value is found by operating in the same way not adding blood as the comparison and the Fe<+2> is measured and then, the quantity of hemoglobin is found by calculating said measured value. |
