HIGH PERFORMANCE IMMOBILIZED ENZYME COMPOSITIONS BY MULTI-LAYERING IMMOBILIZATION OFFERING A HIGH AMOUNT OF ACTIVITY PER UNIT VOLUME

摘要

A high activity immobilized enzyme composite is prepared by covalently bonding a second enzyme layer to a first enzyme layer that is immobilized to u carrier. The process is repeated to apply third, fourth, and more successive enzyme layers. The product has high activity per unit volume, superior stability, and good half-life. Applicable to immunoreactants, hormones, and generally to biologically active material having available amine.