METHOD AND APPARATUS FOR LIQUID CHROMATOGRAPHY

摘要

PURPOSE:To make the mixing property better and the mixing ratio freely adjustable by introducing a plurality of solvents continuously to a specified separation column while the flow rate of transferred phase is controlled with a pump at the downstream of a means for detecting separated individual components of a sample. CONSTITUTION:A solvent A from a variable delivery/constant discharge type pump 1 and a solvent B from a micro-gas syringe 3 of a constant pressure type pump 2 adapted to apply a specified pressure in a system with a loading weight 4 are converged with a tee 9 and delivered to a liquid feed passage 5 while each being refilled from storage tanks 7 and 8 through a three-way cock 6. A sample is introduced from a valve injector 10 on the passage 5 and separated into individual components with a separation column 11 in a transferred phase comprising the solvents A and B. Components of the sample flow out sequentially with the transferred phase and detected with a detector 12. A constant discharge type pump is provided at the downstream of the detector 12 to control of the outgoing from the detector 12.