Coated capillary chromatographic column

摘要

In the production of a wall coated open tubular capillary chromatographic column, improved deactivation of the inner surface of a glass or fused silica capillary tube prior to coating with stationary phase is obtained by a multi-step process whereby siloxane polymer molecules are chemically bonded to the capillary inner surface. The process comprises multiple cycles of successive reactions with silicon tetrachloride and a diorganodichlorosilane vapor with intermediate hydrolyses and a final capping reaction with a triorganochlorosilane. The deactivated surface is adapted to coating with an apolar liquid stationary phase.