| 摘要 |
Recombinant plasmids are described that carry the structural gene for human proinsulin in expressible form enabling for the first time production of human proinsulin by the application of genetic engineerng techniques using a single host organism. The human proinsulin structural gene is fused to a functional transport sequence. Host organisms transformed by the recombinant plasmids express a preproinsulin product that may, depending upon the nature of the host, be correctly processed to human proinsulin in vivo and transported across the host organism's cell wall. Alternatively, expressed human preproinsulin may be recovered from host cells and converted into human proinsulin. Following its preparation in vitro method may be used to convert the human proinsulin to human insulin. |
