| 摘要 |
PURPOSE:To collect the titled substance produced and accumulated in a liquid culture medium, by cultivating a microorganism of the genus Escherichia having the ability to produce the titled substance in the liquid culture medium containing lactose or galactose as a carbon source. CONSTITUTION:A microorganism of the genus Escherichia, e.g. Escherichia coli No.283-5(NRRLB-12295)(tolerant to p-fluorophenylalanine) or Escherichia coil EG-19(NRRLB-12282)[tolerant to S-(2-aminoethyl)-cysteine], having the ability to produce L-glutamic acid is cultivated in a liquid culture medium containing lactose or galactose or milk whey or soybean whey containing them as a carbon source and ammonium ions, phosphoric acid ions vitamins, etc. under aerobic conditions. The aimed L-glutamic acid is then collected from the culture medium by the conventional method. |
