发明名称 Vectors for the insertion therein of foreign DNA fragments, according to any translation phase
摘要 The invention pertains to a set of vectors (or of DNA fragments to be inserted in a vector) which distinguish from one another in that, taking into account one vector in which the number of pairs of bases between the reading initiation point of the vector and a point corresponding to the first pair of bases of a recognition site corresponding to a predetermined restriction enzyme, the two other vectors comprise between the corresponding points additional groups of pairs of bases comprising respectively two pairs of bases on the one hand and either one or four pairs of bases on the other hand, plus possibly whole numbers of triplets. On inserting a determined DNA fragment of which the expression is sought in bacteria in the three vectors, the reading of said DNA fragment will occur in phase as concerns one of the so modified vectors after transfection of bacteria therewith.
申请公布号 US4371625(A) 申请公布日期 1983.02.01
申请号 US19790093270 申请日期 1979.11.13
申请人 PASTEUR INSTITUT;INST NAT SANTE RECH MED 发明人 TIOLLAIS, PIERRE
分类号 C12N15/09;C12N15/00;C12P19/34;(IPC1-7):C12N1/00 主分类号 C12N15/09
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