发明名称 |
Proinsulin genes with modified C-chain. |
摘要 |
Human-like proinsulin gene analogs have been synthesized by a combination of chemical and enzymatic methods. A number of different human-like proinsulin gene analogs with altered C-chains have been designed and can be readily constructed as described. As a part of the strategy, an adaptor for trimming DNA has been used to recover the A-chain insulin gene with the desired sequence from a hybrid plasmid; a related adapter for trimming DNA has been used to shorten the C-chain gene. The synthetic proinsulin gene has been joined to a replicable cloning vehicle and the hybrid DNA transferred to a host cell. The transformed host cells can be grown under selected conditions to yield the proinsulin analogs.
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申请公布号 |
EP0068701(A2) |
申请公布日期 |
1983.01.05 |
申请号 |
EP19820303071 |
申请日期 |
1982.06.14 |
申请人 |
CANADIAN PATENTS AND DEVELOPMENT LIMITED |
发明人 |
NARANG, SARAN A.;WU, RAY J. |
分类号 |
C12N15/09;C07H21/04;C07K14/62;C12N15/66;(IPC1-7):C12N15/00;C12P21/02;C07C103/52 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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