| 摘要 |
PURPOSE:A blood substitute in which an oxygen transporting substance is joined to a polymer, e.g. polyethylene glycol, and hemoglobin with amide linkages. CONSTITUTION:A polymer selected from the group consisting of polyethylene glycol, polypropylene glycol, and ethylene glycolpropylene glycol copolymer is joined to hemoglobin with amide linkages to give an oxygen transporting substance, which is used to give a blood substitute. The hemoglobin to which the polymer is joined by the amide linkages is obtained as follows: A polyether carboxylic acid is reacted with an N-hydroxysuccinimide in the presence of a diayclohexylcarbodiimide in N,N-dimethylformamide, and the resultant cyclohexylurea is filtered and removed. Ether, etc. is added to give an activated ester of the polyether carboxylic acid. the ester is then reacted with hemoglobin or a derivative thereof in an amount of 1-(1/100) that of the ester in an aqueous solution at a pH of 7-9.
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