| 摘要 |
NEW MATERIAL:Plasmid aATM 3 having a molecular weight of about 4.37(+ or -0.05) megadalton, and the number of scission positions by restricted enzyme of one in BamHI, one in Bgl II, three in SalI, and one in XhoI. USE:Usable advantageously as a vector against a microorganism such as Actinomycetes which is important in fermentation industry. The genetic information from Actinomycetes is made into a clone using this plasmid, and it is introduced into the desired microorganism, so that the preparation of a secondary intermediate such as antibiotics, physiologically active substance, etc. caused by Actinomycetes can be increased. Having extremely large copy number per chromosome of 120-160. PROCESS:A bacterium such as Streptomyces castaneoglobisporus IFO 13669 strain belonging to the genus Streptomyces, having plasmid pATM 3, is inoculated into a medium, cultivated, the mold is collected and subjected to bacteriolysis, and plasmid pATM 3 is isolated from the bacteriolysin. |
