Process for the long-term surviving culture of hepatocytes

摘要

A process is disclosed for the long term surviving culture of hepatocytes maintaining their morphological and functional characteristics for long-term-periods, suitable to assest the acute and chronic hepatotoxicity of drugs, chemicals and environmental pollutants. The long term surviving culture of hepatocytes is carried out in a culture media containing fibroblast cells or fibroblast cell products treated to prevent cell multiplication and inoculated under controlled density.