| 摘要 |
NEW MATERIAL:The plasmid pMN1 having a molecular weight of 10.0 mega- dalton and the plasmid pMN2 having a molecular weight of 7.6 mega-dalton, each having kanamycin-resistant gene marker and one incision point to restrictive enzymes EcoRI and SalI, and obtained by integrating the plasmid pSL1 derived from Streptomyces lavendulae to the plasmid pCR1 derived from Escherichia coli. USE:Useful as a vector for the cloning of actinomycetes. The plasmid is also expected to be used as a shuttle vector which can effect the gene cloning in actinomycetes by cloning in Eschericha coli to amplify the gene and then restoring to actinomycetes. PROCESS:pSL1 and pCR1 are incised with SalI, recombined using T4-ligase, inoculated in Escherichia coli, and cultured. Cultured product is selected according to the kanamycin resistance and then to the labelled pSL1, and each one incision point in two incision points by the restrictive enzyme is deleted. |
