| 摘要 |
NEW MATERIAL:A plasmid having the gene concerning in the streptomycin- and/or spectinomycin-resistance, and autonomically replicable in the bacteria belonging to Corynebacterium genus or Brevibacterium genus. EXAMPLE:The plasmid pCG4 having a molecular weight of about 19 mega-dalton. The numbers of the sensitive sites of the plasmid to restriction enzymes are 4, 7, 9, 6 and 6 corresponding to EcoRI, BamHI, HindII, PstI and SalI, respectively. USE:Useful as a clone-forming vector for bacteria belonging to Corynebacterium genus or Brevibacterium genus or species close to the above genera, and as a reagent for the research of recombinant DNA. PROCESS:For example, Corynebaterium glutamicum 225-250 (FERM-P No.5939) is cultured, and the bacterial cells are subjected to the bacteriolysis. The objective plasmid pCG4 can be separated from the bacteriolytic product by conventional method.
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