POLYSACCHARIDE PS-A ISOLATED FROM THE PLANT OF GENUS EPIMEDIUM VIOLACEUM MORR. ET DECNE., PROCESS FOR ITS PREPARATION, AND INFECTION-PREVENTING AGENT AND IMMUNOSTIMULATING AGENT CONTAINING THE PLOYSACCHARIDE AS EFFECTIVE INGREDIENT

摘要

Polysaccharide PS-A isolated from the plant of genus Epimedium violaceum Morr. et decne. having the following properties, process for its preparation, and an infection-preventing agent and an immunostimulating agent containing same as an effective ingredient: 1. Elemental analysis: C=40.92, H=6.17, ash= trace; 2. Molecular Weight: 75,000 + 25,000 (mean molecular weight); 3. Decomposition point: 205oC; 4. pH: 7.0 (100 mg of PS-A in 50 ml of water); 5. Specific rotary power: ¨ADα¨BD19 = -23.6o (in H¿2?O, c = 0.527); 6. IR absorption spectrum: ξ KBr max (cm?-1¿): 3400, 2900, 1620, 1400, 1230, 1060; 7. UV absorption spectrum: no maximum absorptions in 240 to 400 nm; 8. Appearance: white to slightly brown amorphous powder; 9. Solubility: soluble in water, insoluble in methanol, ethanol, acetone, ethyl, acetate, diethyl ether, hexane, and chloroform; 10. Color reaction: positive for the following reactions: (a) anthrone test, (b) Molisch reaction, (c) skatole reaction, (d) Bial reaction; negative for the following reactions: (a) ninhydrin reaction, (b) 2,4-DNP reaction, (c) Selivanoff reaction, (d) naphthoresorcin reaction, (e) carbazole reaction; 11. Constitutive sugar: arabinose and galactose; 12. Uniformity, uniform in ultracentrifugation, electrophoresis, and gel filtration.