摘要 |
Antistreptolysin antibody content of blood or serum samples is determined by contacting a solution of known concentration of oxidised O- streptolysin with a diluted blood or serum sample, allowing the mixture obtained to stand at 15 to 75 DEG C, adding a solution of a reducing agent, waiting for red blood cell sedimentation, checking if haemolysis has occurred, and checking the condition of a control blank. If a serum sample is used, it is necessary to add red blood cells thereto prior to the treatment with the reducing agent. It is possible to use the method for the determination of the antibody threshold, and to continue the method if the preselected threshold has been exceeded. A diagnostic kit containing the reagents necessary for the method is also described. |