PEROXIDASE DETERMINATION BY EMISSION OF LIGHT

摘要

The present invention provides a process for the determination of peroxidase in the presence of a compound, the enzyme-catalysed oxidation of which with a peroxy compound results in the emission of light, which is measured, wherein 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide is used as light-emitting compound. The present invention also provides a reagent for the determination of peroxidase, comprising 7-dimethyl-aminonaphthalene-1,2-dicarboxylic acid hydrazide, a system providing hydrogen peroxide and a buffer substance of pH 6 to 9.