发明名称 |
Diagnostic test for hepatitis B virus. |
摘要 |
<p>A method and test kit are disclosed for detecting the presence of hepatitis B virus in a test specimen containing at least a portion of the DNA of the virus. A test reagent comprises cloned hepatitis B virus-DNA that has been repurified by treatment with a restriction enzyme and labelled to high specific activity with a radioactive label. The sample to be tested is fixed to a solid matrix, incubated in the presence of the test reagent under hybridization conditions and detected by hybridization to the labelled DNA probe. The uncombined HBV-DNA (labelled) is removed from the substrate, and the hybridized HBV-DNA determined by scintillation counting or by autoradiography of the substrate.</p> |
申请公布号 |
EP0062286(A1) |
申请公布日期 |
1982.10.13 |
申请号 |
EP19820102673 |
申请日期 |
1982.03.30 |
申请人 |
ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY |
发明人 |
SHAFRITZ, DAVID ANDREW |
分类号 |
C07K14/02;C12Q1/70;(IPC1-7):12Q1/70;01N33/50 |
主分类号 |
C07K14/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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