| 摘要 |
NEW MATERIAL:Trisarubicinol (acid addition salt) of formulaIhaving the following physical and chemical properties: appearance, dark red powder; melting point, 149-152 deg.C; molecular weight, 785.84; elemental analysis, C 60.62, H 6.47, N 1.93; optical rotation,[alpha]D<23>=+1.84 deg.(C=0.8, CHCl3), etc. USE:Low-toxic antitumor agent. PROCESS:A bacterial strain such as Streptomyces galilaeus KE 303 (ATCC 31649, FERM-P No.4808), is cultured preferably in a medium, at 25-32 deg.C for 1-3 days under shaking. The produced seed strain is inoculated in a conventional liquid medium, and cultured for 15-48hr. A methanol solution of anthracyclinone of formula II[R is COCH2 or CH(OH)CH3]is added to the medium to the final concentration of 10-200mug/ml, and the cultivation is continued for 15- 72hr. |
