Systems and Methods for Using Interleaving Window Widths in Tandem Mass Spectrometry

摘要

Systems and methods are provided for analyzing a sample using overlapping measured mass selection window widths. A mass range of a sample is divided into two or more target mass selection window widths using a processor. The two or more target widths can have the same width or variable widths. A tandem mass spectrometer is instructed to perform two or more fragmentation scans across the mass range using the processor. Each fragmentation scan of the two or more fragmentation scans includes a measured mass selection window width. The two or more measured widths of the two or more fragmentation scans can have the same width or variable widths. At least two of the two or more measured mass selection window widths overlap. The overlap in measured mass selection window widths corresponds to at least one target mass selection window width.

主权项

1. A system for analyzing a sample using overlapping measured mass selection window widths, comprising:
a tandem mass spectrometer that includes a mass analyzer that allows overlapping measured mass selection window widths; and a controller in communication with the tandem mass spectrometer that is
configured to divide a mass range of a sample into two or more target mass selection window widths, andconfigured to instruct the tandem mass spectrometer to perform two or more fragmentation scans across the mass range, wherein each fragmentation scan of the two or more fragmentation scans comprises a measured mass selection window width and wherein an overlap in measured mass selection window widths of at least two fragmentation scans of the two or more fragmentation scans corresponds to at least one target mass selection window width of the two or more target mass selection window widths.