Method for the separation of a protein substance from a solution containing the same by affinity filtration and application of said method to enzymatic assays

摘要

The invention relates to a method for the selective separation of a given protein substance having an affinity for a ligand from other substances some of them having the same affinity for the ligand but also having a high molecular weight, higher than that of said protein substance, said substances being in solution. The method consists of filtering said substances on a gel which excludes said high molecular weight substances, said gel being coupled with a ligand for which said protein substance has affinity. The method may be applied to enzymatic and especially to immunoenzymatic assays.