VERFAHREN ZUR HERSTELLUNG VON AMINOGESCHUETZTEN L-ASPARTYL-L-PHENYLALANIN-ALKYLESTERN

摘要

A process for the production of amino-protected-L-aspartyl-L-phenylalanine alkyl ester addition compound of the formula in which R1 represents an amino protecting group and R2 is an alkyl group containing from 1 to 6 carbon atoms, which comprises reacting a carboxyl- protected-L-phenylalanine of the formula phi -CH2- in which R2 has the above-stated meaning in the presence of a protease producing microorganism or an enzyme obtained from a microorganism having protease activity under conditions which favour microbial enzyme coupling in the presence of an enzyme at a pH which maintains enzyme activity, to form an amino-protected-L-aspartyl-L-phenylalanine alkyl ester addition compound of the formula and continuously removing said alkyl ester addition product. Compounds such as L-aspartyl-L-phenylanine methyl ester may be prepared from the amino-protected alkyl esters by removal of the protecting group, by catalytic hydrogenation.