| 摘要 |
S-Acetylglutathione is manufactured by treating glutathione with acetic anhydride in acetic acid solution in the presence of perchloric acid. Advantageously the glutathione is dissolved in a mixture of glacial acetic acid and perchloric acid before adding acetic anhydride, preferably in molar excess, e.g. of about 40-70 per cent. The perchloric acid may be added in the form of an aqueous solution of not less than about 70 per cent w/w concentration, and preferably in an amount sufficient to give a concentration in the reaction mixture of about 0.5-1.0 molar. The reaction is preferably effected below about 25 DEG C. with cooling as necessary, and on its completion the excess of acetic anhydride may be decomposed with water and the perchloric acid neutralized (e.g. by adding aniline in about 30 per cent excess over the amount theoretically required) before isolating the product, e.g. by extraction with a liquid such as methanol from which it will crystallize on standing. Detailed examples are given in which 72 per cent w/w aqueous perchloric acid is used. |
