ALPHA-AMINO-EPSILON-CAPROLACTAM RACEMASE

摘要

PURPOSE:To improve the productivity of a microbial strain capable of producing alpha-amino-epsilon-caprolactam (ACL), by producing an ACL racemase having a specific amino acid sequence and a DNA sequence coding said racemase. CONSTITUTION:A gene DNA coding alpha-amino-epsilon-caprolactam (ACL) racemase is produced from a chromosome DNA of Achromobacter oobae (FERM P-776) or from a composite plasmid DNN36. A recombinant E.coli transformed with a composite plasmid DNN40 having structural gene of Achromobacter oobae or enzyme can be used as a bacterial strain capable of producing ACL racemase. The objective ACL racemase can be easily extracted from the recombinant E.coli and purified.