摘要 |
A process for the production of fats and oils, and particularly fats and oils rich in triglycerides, and used as cocoa butter substitutes, comprising cultivating yeast cells capable of synthesizing the fats and oils in a medium containing an emulsion of at least one fatty acid having 10 to 20 carbon atoms, followed by separation of the fats and oils from the cultivated cells. The emulsion is preferably formed by heating the fatty acid with a buffer, adding an emulsifier, homogenising and rapidly cooling to produce an emulsion containing stearic acid particles of 10 microns or less. The medium may also contain carbon and nitrogen nutrients and desaturase enzyme inhibitor (if low levels of unsaturated acids are required). |