| 摘要 |
PURPOSE:A film of one layer - four layers for measuring quantitatively transaminase activity, consisting of a transaminase substrate, pyruvic oxidase, a compound for source of phosphoric acid, metal ion, a color indicator composition for detecting hydrogen peroxide, etc. CONSTITUTION:On the sheet-shaped substrate 10, the reaction layer 22 comprising three of a transaminase substrate, pyruvic oxidase, and a color indicator composition for detecting hudrogen peroxide, the dye fixed layer 24 containing an anionic polymer, the light reflecting layer 40, and porous developing layer are formed. The color indicator composition for detecting hydrogen peroxide consists of a substance having a peroxidase action, a hydrogen donor, and a coupler, and this combination can form a cationic dye. The color indicator layer may consists of one, two, or three layers. A cationic dye having high absorbance formed by the color indicator for detecting hydrogen peroxide is subjected to colorimetric measurement to determine activity.
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