发明名称 |
Method for preparing a recombinant DNA phage |
摘要 |
A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.
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申请公布号 |
US4348477(A) |
申请公布日期 |
1982.09.07 |
申请号 |
US19790087166 |
申请日期 |
1979.10.22 |
申请人 |
NODA INSTITUTE FOR SCIENTIFIC RESEARCH |
发明人 |
NAKANO, EIICHI;MASUDA, TSUTOMU;SAITO, NARIMASA;FUKUSHIMA, DANJI |
分类号 |
C12N15/09;C12N15/00;C12P19/34;C12R1/91;(IPC1-7):C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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