发明名称 Method for preparing a recombinant DNA phage
摘要 A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.
申请公布号 US4348477(A) 申请公布日期 1982.09.07
申请号 US19790087166 申请日期 1979.10.22
申请人 NODA INSTITUTE FOR SCIENTIFIC RESEARCH 发明人 NAKANO, EIICHI;MASUDA, TSUTOMU;SAITO, NARIMASA;FUKUSHIMA, DANJI
分类号 C12N15/09;C12N15/00;C12P19/34;C12R1/91;(IPC1-7):C12N15/00 主分类号 C12N15/09
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