摘要 |
<p>A method for the determination of peroxide in a sample is disclosed which comprises reacting the peroxide with a chromogen represented by the general formula (I) or (II) general formula (I): <IMAGE> general formula (II): <IMAGE> wherein R1 and R3 represent amino, mono- or di- substituted amino, hydroxyl or hydroxyalkyl, R4 and R5 represent hydrogen, alkyl, alkylene, acyl, halogen, sulphone, nitro, carboxyl, hydroxyl or hydroxyalkyl, R2 represents hydrogen, <IMAGE> wherein R6 represents hydrogen, alkyl, aralkyl, alkylene, aryl or mono- or di- substituted aryl, and -Z- may change to -Z= by resonance and represents -S-, -O-, -N=, <IMAGE> wherein R7, R8, R9 and R10 have the same significance as R6 in the presence of heme compound, iodide or bromide and measuring the absorbancy of the reaction solution in the visible ray region. Also disclosed is a test composition for the determination of peroxide which comprises a chromogen as defined above and a compound selected from a heme compound, iodide and bromide.</p> |