发明名称 |
DNA TRANSFER VECTOR AND MICRO-ORGANISM MODIFIED TO CONTAIN A NUCLEOTIDE SEQUENCE EQUIVALENT TO THE GENE OF A HIGHER ORGANISM |
摘要 |
New DNA transfer vector contains in its nucleotide sequence a sub-sequence having the structure of and transcribes from, a gene of a higher organism. Specifically the sub-sequence is the code for human insulin A or B chain (or both), or human growth hormone (the nucleotide sequences of these codes are given). Also new are microorganisms including in their nucleotide structure such as a sub-structure. - The microorganisms are made by first isolating an appropriate messenger-RNA; purifying it, then using it to elaborate a double-strand complementary DNA (c-DNA) contg. the required code. This is then reacted with a DNA-transfer vector having reactive end-groups able to react with itself or with the c-DNA. - The modified microorganisms can be used to produce insulin or growth hormone by fermentation, which is easier and quicker than cultivation of cell lines and gives better yields.
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申请公布号 |
NZ187300(A) |
申请公布日期 |
1982.08.17 |
申请号 |
NZ19780187300 |
申请日期 |
1978.05.17 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
RUTTER W;GOODMAN H;CHIRGWIN J;ULLRICH A;PICTET R;SEEBURG P;SHINE J |
分类号 |
C12N15/09;C07K14/575;C07K14/62;C12N1/00;C12N1/16;C12N1/21;C12N15/00;C12N15/10;C12P19/34;C12P21/00;C12P21/02;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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地址 |
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