摘要 |
A method and apparatus for the determination of the concentration of certain chemical substances such as glucose, urea, enzymes, etc. in biological solutions such as blood, serum, urine, using a technique of differential pH measurement using two pH electrodes, and measuring pH changes in the solutions after the addition of specific reagents, the concentration being determined according to the formula [sample]=FCALx( DELTA pHc- DELTA pHb- DELTA pHo)wherein [sample] is the concentration of the substance in the sample, FCAL is a calibration factor, DELTA pHc is the measured pH of the reacted sample, DELTA pHb is a pH correction for the reaction initiating compound, and DELTA pHo is a correction for background pH changes. |