摘要 |
A process for the production of fats and oils, and particularly fats and oils rich in triglycerides and used as cocoa butter substitutes, comprising (a) cultivating yeast cells capable of synthesizing the desired fats and oils to promote growth in a growth medium containing carbon and nitrogen nutrients such that the cells are grown in their logarithmic phase and then harvesting and (b) cultivating the harvested yeast cells in a lipid accumulation medium, preferably an emulsion, including at least one fatty acid containing 10 to 20 carbon atoms. The two-stage process produced greater yield of fats as the first stage requires a high nitrogen:carbon ratio (2 or more:1) and the stage requires a high carbon:nitrogen ratio (10 or more:1). |