METHOD OF SELECTIVELY INCREASING YIELD AND PURITY OF LOW TEMPERATURE PRECIPITATION PROTEIN

摘要

AHF and Clg yield and purity in cryoprecipitate concentrate are improved by suspending said cryoprecipitate concentrate in aqueous solution and then subjecting to a heat treatment step at a temperature of from about 45 DEG C to about 60 DEG C for a period of from about one minute to about sixty minutes to thereby selectively thermally precipitate the contaminating proteins without substantial loss of AHF or Clg activities and recovering the purified AHF or Clg from the precipitate of contaminating proteins.